Fill about 2.5 ml culture medium into a well of a 24-well culture plate (23). Then place the tissue carrier with a forceps into the well (24). Please turn the carrier within
the medium and have a look, that gas bubbles are not attached to the carrier. Soak off the medium by a pipet until the level of fluid has reached the upper surface of the
carrier (25). Not more than 1 mm in height culture medium is now present in the upper compartment of the tissue carrier. Then pipet your cells or place a piece of tissue
onto the upper surface of the carrier (26).
Culture with 1 cell type: In the case you like to culture one cell type please place the tissue carrier with the white ring on top and black ring towards the bottom of the culture plate.
Co-culture with 2 cell types: Please start with the white ring on the bottom of the culture plate. Settle your first cell type until adhesion and turn then the tissue carrier to settle the second cell type.