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MINUCELLS AND MINUTISSUE VERTRIEBS GMBH

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PAGE 05

The cell support is placed in a MINUSHEET carrier for further handling:

An optimal support for cells and tissues is selected to improve the anchorage and thereby the differentiation under in vitro conditions. Numerous investigations showed that the cellular phenotype is drastically influenced by the selection of an optimal, suboptimal or unsuitable support. Since each cell type reacts in a different way, the optimal support has to be elaborated individually. See references in proceedings.

part Nr. 1300
part Nr. 1300

The selection of an optimal cell adhesion support, resulting in high differentation, becomes possible within a MINUSHEET. It consists of a black base ring, in which the selected support material is placed. Commercially available supports such as glass, foils, filters, nets, fleeces or biological membranes with a diameter of 13 mm can be used. A white tension ring is used to hold the support in place on the base ring. Finally the carrier arrangement is autoclaved or sterilized in ethanol or formaline depending of the support. See references in proceedings.

part Nr. 1300
part Nr. 1300

For further use the NINUSHEET carriers are placed in a conventional culture dish with fine forceps. Then the cells are transferred within a droplet of medium to the surface of the support. Tissue slices can be placed between two nylon nets just like a sandwich.

The following items are necessary for an experiment:

1 x Nr. 1300
6 pieces MINUSHEETS for 13 mm supports
part Nr. 1300
part Nr. 1300

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