Culture conditions as close as
possible to the situation within
an organ:


The value of cultured cells and tissues in biomedical research is highly dependent on their degree of terminal differentiation. In the stagnant environment of conventional culture dishes however, cells and tissues lose their morphological, physiological and biochemical features by dedifferentiation. As a result they are not comparable to the situation within in an organ any more. Because many experiments failed in conventional dishes, we developed a new technique to obtain improved differentiation.

We are proud that our project was awarded by the Philip Morris research prize "Challenge of the future" in 1992.

MINUSHEET is a registered trade mark.

The increasing demand for our tools finally was the reason for Katharina Lorenz-Minuth to found a private company in 1993.

From numerous experiments we know that optimal differentiation can only be obtained, if the environment is adapted as close to the natural situation as possible. Furthermore we experienced that differentiation of cells and tissues in vitro depends not only on a single morphogenic substance but on several environmental factors.

Cells and tissues are kept on interchangeable supports mounted in special carriers, the MINUSHEEETS. This way an ideal support for the cells can be selected. Optimal support and cell adhesion is an important presuppostion for differentiation.

The cell and tissue carriers can be used in conventional tissue culture plates or they are placed in newly constructed per(i)fusion culture containers. A continuous flow of culture medium guarantees optimal nutrition, prevents an overshoot of paracrine factors and keeps harmfull metabolic products on a constantly low level.

The developed per(i)fusion culture system is based on the following patents:

DE 10 2004 054 125,
DE 39 23 279,
DE 42 00 446,
DE 42 08 805,
DE 44 43 902,
DE 19 530 556,
DE 196 48 876 C2,
DE 199 52 847 B4,
US 5 190 878,
US 5 316 945,
US 5 665 599,
J 2847669,
DE 10 2005 002 938,
PA 10 2005 001 747.9,
PA 10 2004 054 125.6
and others.

For ecological reasons all of the parts can be autoclaved and are constructed for numerous repeated use.

The advanced culture system was developed by Will W. Minuth and his team at the Department of Anatomy, University of Regensburg, Regensburg, Germany. This was the reason why many researchers started asking for the system.

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